Expressão da proteína quinase ativada por AMP e sirtuina 1 em pacientes submetidos à cirurgia bariátrica

Abstract

Objective: to evaluate the expression of AMP-activated protein kinase (AMPK) and Sirtuin-1 (SIRT-1) in liver tissue samples from patients during bariatric surgery and one year after the procedure. To evaluate the association between the expression of these proteins with weight, Body Mass Index (BMI) and biochemical parameters (glucose, total cholesterol, HDL and triglycerides). Methods: the expression of the proteins was verified by immunohistochemistry and quantified by digital image analysis. All other data were accessed in the database. Results: liver biopsies of 44 patients submitted to bariatric surgery were analyzed. 31 (70.5%) were women and 13 (29.5%) were men. The mean age was 37.18 ± 11.26 years. The mean preoperative weight was 127.29 ± 27.10kg, decreasing to 82.00 ± 19.94kg postoperatively. With this significant reduction, the mean BMI also reduced by removing the patient's classification from obesity grade III to overweight. The mean biochemical parameters also showed significant improvement, with the exception of HDL, which showed no significant change. SIRT-1 expression had a mean value of 128.80μm2 in the intraoperative biopsies and 306.68μm2 in the biopsies performed one year after (p = 0.0069). And AMPK had mean values of 4166.10μm2 and 2718.70μm2 respectively (p = 0.0028). Conclusion: the present study showed an increased expression of SIRT-1 and a decrease in the expression of AMPK in postoperative liver biopsies. This pattern of expression accompanied improvements in weight, BMI and the profile of biomarkers that are associated with the development of co-morbidities also verified one year after surgery.