Citoxicidade dos materiais protéticos dentários utilizados em implantodontia : estudo in vitro

Abstract

The choice of material used in the manufacture of dental prostheses on implants can be directly linked to the quality and maintenance of gingival tissue. The aim of this study was to evaluate the cytotoxicity of dental prosthetic materials in fibroblasts in cell culture by MTT assay at 24, 48, 72 h. Samples of materials were prepared: Titanium (Ti), polietereter-ketone (PEEK), composite resin (CR) and acrylic resin (AR) and left in contact with D-MEM for 24 h. NIH/3T3 cells was seeded in 96-well plate and added to the medium that were in contact with the test material. Wells containings only cells were used for negative control (NC) for toxicity. For positive control (PC) for toxicity cells were subjected to the action of sodium hypochlorite. The results were subjeted to calculating the percentage of cell viability and to statistical one-way anlysis of variance (ANOVA) followed by Tukey test (p <0.05). The results of percentage cell viability at 24 h were CN 100%, Ti 67.52%, 65.71% PEEK, RC 44.49%, RA 49.09%, CP 4.29%. Conclusion: The Ti and PEEK demonstrated a similar trend in our research, as well as the RC and RA. The worst results were observed for RC at all times observed.