Incorporação do hormônio do crescimento humano recombinante (rhGH) em matriz de polímero biodegradável

Abstract

Objective: To incorporate recombinant human growth hormone in a biodegradable polymer (PLGA) Material and Methods: It was used a blend of two solvents, methanol and dichloromethane in a ratio by weight of 1.5: 5.0 for dissolving the PLGA. Another solution was made with rhGH, zinc acetate and deionized water. The two mixtures were then mixed together under mechanical stirring and poured into silicone molds circular of 01cm in diameter and around 02mm of espessur. This mixture was left in the evaporation chamber of solvent for 48 hours. The matrices were then degraded in vitro in PBS pH 7.4, thermostatted bath. The samples were removed from the bath in the range of 01, 02, 03, 04, 07, 10, 14 days. Was measured weight loss, pH and concentration of hormone released versus time. Results: The concentration of hormone released versus time was increased until the third day. On the fourth day had a fall and on the seventh day there have been increased hormone released by the tenth day, the fourteenth day was falling again. The pH had a sharp drop from 7.4 to 3.2 on the first day and keeping a small drop until the fourteenth day. The mass loss was a gradual loss in relation to time as was to be expected. Conclusion: The PLGA is a good biomaterial for making this type of device. You can incorporate the rhGH this array to get a possible material that may serve as a bone graft.